Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine
Article Title: Dual-labeled trastuzumab-based imaging agent for the detection of human epidermal growth factor receptor 2 overexpression in breast cancer.
doi: 10.2967/jnumed.107.042234
Figure Lengend Snippet: FIGURE 5. Athymic mice bearing SKBr3-luc xenografts were sacrificed at 5, 24, 48, and 72 h after intravenous injection of (111In-DTPA)n-trastuzumab-(IRDye800)m, and major organs were dissected. (A) Notched box-and-whisker statistical plots compare TMRs obtained with g-scintillation counting for nuclear counts and NIR fluorescence imaging for optical counts at various time points. There was no statistical significance in TMRs observed at various time points within each imaging modality, but TMRs obtained with optical imaging were statis- tically higher than those obtained with nuclear imaging (P , 0.0001) at each time point. (B) Plots of nuclear versus optical TMRs obtained with nuclear imaging and optical imaging for these mice yielded an R value of 0.9476 (P , 0.0001), suggesting that results obtained from nuclear imaging and optical imaging are highly correlated. (C) When TMRs were compared on a consistent basis with respect to tissue weight (g), the statistical differences between optical and nuclear imaging are abolished.
Article Snippet: Cells were suspended in 100 mL of PBS and added to a microtiter plate, and the NIR fluorescence signal was obtained by use of an Odyssey infrared imaging system (Li-Cor Biosciences).
Techniques: Injection, Whisker Assay, Imaging, Optical Imaging